Immune responses and histopathological changes in rabbits immunized with inactivated SARS coronavirus
Identifieur interne : 003094 ( Main/Exploration ); précédent : 003093; suivant : 003095Immune responses and histopathological changes in rabbits immunized with inactivated SARS coronavirus
Auteurs : Chuan-Hai Zhang [République populaire de Chine] ; Xin-Jian Liu [République populaire de Chine] ; Yi-Fei Wang [République populaire de Chine] ; Jia-Hai Lu [République populaire de Chine] ; Huan-Ying Zheng [République populaire de Chine] ; Sheng Xiong [République populaire de Chine] ; Mei-Ying Zhang [République populaire de Chine] ; Qiu-Ying Liu [République populaire de Chine]Source :
- Virologica Sinica [ 1674-0769 ] ; 2008.
Abstract
To evaluate the immunogenicity of inactivated SARS coronavirus (SARS-CoV), three groups of rabbits were immunized three times at 2-week intervals with inactivated vaccine + adjuvant, adjuvant, and normal saline respectively. Eight batchs of serum were sampled from the auricular vein at day 7 to day 51, and specific IgG antibody titers and neutralizing antibody titers were detected by indirect ELISA and micro-cytopathic effect neutralizing test. Antibody specificity was identified by proteinchip assay. Histopathological changes were detected by H&E staining. The results showed that, rabbits in the experimental group immunized with inactivated SARS-CoV all generated specific IgG antibodies with neutralizing activity, which suggested the inactivated SARS-CoV could preserve its antigenicity well and elicit an effective humoral immune responses. The peak titer value of specific IgG antibody and neutralizing antibody reached 1:40960 and 1:2560 respectively. In the experimental group, no obvious histopathological changes was detected in the H&E stained slides of heart, spleen, kidney and testis samples, but the livers had slight histopathological changes, and the lungs presented remarkable histopathological changes. These findings are of importance for SARS-CoV inactivated vaccine development.
Url:
DOI: 10.1007/s12250-007-0033-0
PubMed: NONE
PubMed Central: 7090504
Affiliations:
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Chine</country><wicri:regionArea>Guangzhou</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Qiu Ying" sort="Liu, Qiu Ying" uniqKey="Liu Q" first="Qiu-Ying" last="Liu">Qiu-Ying Liu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.258164.c</institution-id><institution-id institution-id-type="ISNI">0000000417903548</institution-id><institution>Biomedicine Research & Development Center,</institution><institution>Jinan University,</institution></institution-wrap>Guangzhou, 510630 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangzhou</wicri:regionArea></affiliation></author></analytic><series><title level="j">Virologica Sinica</title><idno type="ISSN">1674-0769</idno><idno type="eISSN">1995-820X</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>To evaluate the immunogenicity of inactivated SARS coronavirus (SARS-CoV), three groups of rabbits were immunized three times at 2-week intervals with inactivated vaccine + adjuvant, adjuvant, and normal saline respectively. Eight batchs of serum were sampled from the auricular vein at day 7 to day 51, and specific IgG antibody titers and neutralizing antibody titers were detected by indirect ELISA and micro-cytopathic effect neutralizing test. Antibody specificity was identified by proteinchip assay. Histopathological changes were detected by H&E staining. The results showed that, rabbits in the experimental group immunized with inactivated SARS-CoV all generated specific IgG antibodies with neutralizing activity, which suggested the inactivated SARS-CoV could preserve its antigenicity well and elicit an effective humoral immune responses. The peak titer value of specific IgG antibody and neutralizing antibody reached 1:40960 and 1:2560 respectively. In the experimental group, no obvious histopathological changes was detected in the H&E stained slides of heart, spleen, kidney and testis samples, but the livers had slight histopathological changes, and the lungs presented remarkable histopathological changes. These findings are of importance for SARS-CoV inactivated vaccine development.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cheng, M" uniqKey="Cheng M">M. Cheng</name></author><author><name sortKey="Chan, C W" uniqKey="Chan C">C. W. Chan</name></author><author><name sortKey="Cheung, R C" uniqKey="Cheung R">R. C. Cheung</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W. 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Zhang</name></author><author><name sortKey="Lu, J H" uniqKey="Lu J">J. H. Lu</name></author><author><name sortKey="Wang, Y F" uniqKey="Wang Y">Y. F. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, C H" uniqKey="Zhang C">C. H. Zhang</name></author><author><name sortKey="Wang, Y F" uniqKey="Wang Y">Y. F. Wang</name></author><author><name sortKey="Zhang, M Y" uniqKey="Zhang M">M. Y. Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, X" uniqKey="Zhang X">X. Zhang</name></author><author><name sortKey="Li, H" uniqKey="Li H">H. Li</name></author><author><name sortKey="Zheng, K" uniqKey="Zheng K">K. Zheng</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Chuan Hai" sort="Zhang, Chuan Hai" uniqKey="Zhang C" first="Chuan-Hai" last="Zhang">Chuan-Hai Zhang</name></noRegion><name sortKey="Liu, Qiu Ying" sort="Liu, Qiu Ying" uniqKey="Liu Q" first="Qiu-Ying" last="Liu">Qiu-Ying Liu</name><name sortKey="Liu, Xin Jian" sort="Liu, Xin Jian" uniqKey="Liu X" first="Xin-Jian" last="Liu">Xin-Jian Liu</name><name sortKey="Liu, Xin Jian" sort="Liu, Xin Jian" uniqKey="Liu X" first="Xin-Jian" last="Liu">Xin-Jian Liu</name><name sortKey="Lu, Jia Hai" sort="Lu, Jia Hai" uniqKey="Lu J" first="Jia-Hai" last="Lu">Jia-Hai Lu</name><name sortKey="Wang, Yi Fei" sort="Wang, Yi Fei" uniqKey="Wang Y" first="Yi-Fei" last="Wang">Yi-Fei Wang</name><name sortKey="Xiong, Sheng" sort="Xiong, Sheng" uniqKey="Xiong S" first="Sheng" last="Xiong">Sheng Xiong</name><name sortKey="Zhang, Chuan Hai" sort="Zhang, Chuan Hai" uniqKey="Zhang C" first="Chuan-Hai" last="Zhang">Chuan-Hai Zhang</name><name sortKey="Zhang, Mei Ying" sort="Zhang, Mei Ying" uniqKey="Zhang M" first="Mei-Ying" last="Zhang">Mei-Ying Zhang</name><name sortKey="Zheng, Huan Ying" sort="Zheng, Huan Ying" uniqKey="Zheng H" first="Huan-Ying" last="Zheng">Huan-Ying Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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